Production methods of microorganisms have evolved that allow for modification or generation of metabolic pathways

摘要

The present invention relates to a novel process for the preparation of microorganisms have evolved that allow modification of metabolic pathways, the method is characterized in that it comprises the following steps: such as inhibiting the production or consumption of a) metabolite A process for producing a modified microorganism by the purposes, the genetically engineered microbial cells of the first step of the microorganism is cultured in effect defined media is also for the growth ability of the microbe, b at this time the method comprising culturing a modified microorganism obtained as described above in the defined medium to induce evolution in) the cell, and adding a co-substrate in the defined medium to allow the of this time The step of selecting the cells of the modified microorganism can be grown in a defined medium with good co-substrate process, c) optionally even need to be. The present invention evolved genes encoding evolving proteins obtainable strain by the method of evolving microorganisms obtained in this way, and also relates to the use of protein of microorganisms in the biological conversion method, or a gene.