And executed by a computer target base sequence search method of RNA interference, the base sequence design method of a polynucleotide that causes RNA interference, a method for manufacturing a double-stranded polynucleotide, method of inhibiting the expression of a gene, the base sequence processing apparatus, the base sequence processing method program to the recording medium, and, the base sequence processing system

摘要

It is interfered from the RNA of above-mentioned base sequence target gene, Sequence meets detection claimed below. It is based on detection as a result, make RNAi siRNA design, synthesis and etc.. It (one) is adenine, thymidine or uracil at 3 end of pedestal. It (two) is guanine or cytimidine at 5 end of pedestal. (3) there are the base sequence 3 end bases of 7 bases to be selected from adenine, thymine and uracil rich in one or more. (being used as in step d) there are number as alkali causes RNA interference without showing any cytotoxicity.