Dual expression vector system for antibody expression in mammalian cells and bacterial cells

摘要

Dual expression vector, using the expression and secretion of the full-length polypeptide of interest in eukaryotic cells, and the invention provides a method for the expression and secretion of a soluble fragment or domain of the polypeptide in bacteria. When expressed in bacteria, by transferring the bacterial promoters of the first intron, and terminates at the stop codon of the second intron, a fragment of a polypeptide, such as Fab fragments are expressed. In mammalian cells, bacterial regulatory sequences located within introns of the two splicing is removed, mammalian signal sequences may be generated, full-length polypeptide, for example, a light chain polypeptide or an IgG heavy chain is expressed by this contrast to. Dual expression vector system of the present invention may be used for screening and selecting a new monoclonal antibodies, as well as to optimize the monoclonal antibodies for binding to the antigen molecule of interest.