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High-throughput proteomics and a method for binding Zyme array
High-throughput proteomics and a method for binding Zyme array
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机译:高通量蛋白质组学和结合Zyme阵列的方法
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摘要
The provided herein how to create a corresponding high-throughput screening to identify quickly the variations of one or more polypeptides in a biological sample and different methods of identifying the absence or polypeptide variations present in a biological sample between different . Specifically, for example, it is possible to identify variations in post-translational modifications of the polypeptide on a particular biological sample such as the presence or absence of a polypeptide phosphoryl moiety is attached. In these methods, it is utilized as a substrate-specific binding protein (ie, binding zyme) a catalytically inactive enzyme. Binding DNAzyme These can be combined with one or more substrates in a biological sample, it can serve as a marker to distinguish from those of another sample that is bound substrate. Isolation of substrates for their identification, detection of substrate in a sample, and methods are also useful in the discovery and development of medical drugs.
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