Meiotic arrest of human oocytes in vitro

摘要

The method for in vitro synchronisation of nuclear and cytoplasmatic maturation of GV oocytes from domestic animals or from primates can be improved if a phosphodiesterase type 3 inhibitor is added to the medium after collection of the oocytes and, thereafter, said phosphodiesterase type 3 inhibitor is removed to allow the nuclear maturation to proceed.