DNA polymerase having a base analog detection activity decreased

摘要

The present invention relates to the generation and analysis of archaeal DNA polymerase mutant having a base analog detection activity decreased. The present invention is an archaeal DNA polymerase mutant with containing additional mutations that modulates the DNA polymerase activity of the other, including the 3'-5 'exonuclease activity or DNA polymerase, a base analog detection activity decreased provide. The present invention also, I disclose the methods and applications of DNA polymerase with base analog detection activity was reduced.