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Nuclear transplantation egg, production method of parthenogenesis embryo and parthenogenesis mammal

机译:核移植卵,单性生殖胚胎的生产方法和单性生殖哺乳动物

摘要

This invention, the nuclear transplantation egg which 2 sets it possesses the half genome of ovum origin of the mammal, the parthenogenesis embryo, designates that production method of the parthenogenesis mammal is offered as purpose. Acquired gene decoration is received with about spermatogenesis retreating/quitting in the method of producing the nuclear transplantation egg where (1) after introducing the ng ovum into the GV period egg which is pited, external it matures cultures this invention and, moves to MII period and the process which is made the 1st nuclear transplantation egg, (2) it removes MII period chromosome from the said 1st nuclear transplantation egg, another MII period egg (the fg ovum) introduces and consists of the process which is made the 2nd nuclear transplantation egg, possesses the haploid genome set of ng ovum origin and the haploid genome set of fg ovum origin, as a ng ovum or a fg ovum,It features that the ovum which the imprint gene deficiency is done is used, the nuclear transplantation egg, the parthenogenesis embryo, it is production method of the parthenogenesis mammal.
机译:本发明的2套核移植卵具有哺乳动物卵子起源的半基因组,孤雌生殖胚胎,旨在提供孤雌生殖哺乳动物的生产方法。在制造核移植卵的方法中,伴随精子发生的后退/退出而获得获得的基因修饰,其中(1)将ng卵子注入到去核的GV期卵中,使其成熟后培养,进入MII期。以及作为第一核移植卵的过程,(2)从所述第一核移植卵中去除MII期染色体,再引入另一个MII时期卵(fg卵),并由第二核移植过程组成卵具有ng卵子或fg卵子,具有ng卵子起源的单倍体基因组和fg卵子起源的单倍体基因组,其特征在于使用了印迹基因缺失的卵子,核移植卵,单性生殖胚胎,是单性生殖哺乳动物的生产方法。

著录项

  • 公开/公告号JPWO2005011371A1

    专利类型

  • 公开/公告日2006-09-14

    原文格式PDF

  • 申请/专利权人 学校法人東京農業大学;

    申请/专利号JP20050512613

  • 发明设计人 河野 友宏;尾畑 やよい;

    申请日2004-08-04

  • 分类号A01K67/027;C12N15/09;C12N5/10;

  • 国家 JP

  • 入库时间 2022-08-21 21:50:05

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