Growth of human dendritic cells for cancer immunotherapy in closed system using microcarrier beads

摘要

A method and apparatus for reproducibly generating dendritic cells are provided. Blood mononuclear cells are loaded into a cell culture container containing microcarrier beads therein. Tissue culture comprising the cells loaded in the container is incubated for a predetermined period. Nonadherent cells and cells adhered to the beads are separated. Dendritic cell culture medium may be prepared and transferred to the container after the cells which adhere to the beads are separated from the nonadherent cells. The tissue culture incubated for the predetermined time period may be washed to remove nonadherent cells. The beads may be allowed to settle and supernatant is expressed off. The container may comprise a gas permeable cell culture bag.