Novel method for separation of human sperm from biological samples for application in human identification

摘要

The development of an isolation methodology for separation of human sperm cells from biological samples containing human epithelial cells is provided. Using sperm binding proteins, glycopeptides, lectins, derivatives of N-acetylglucosamine, triazine dyes or inhibitors of glycosyltransferase linked to an insoluble support, our invention enables binding of human sperm cells from biological samples. Bound sperms can be dissociated and used for in vitro analyses or subsequently lysed on the insoluble support for isolation of male specific DNA. Other cell types such as epithelial cells, white blood cells and cell debris present in the biological samples are not bound to the derivatized insoluble support. The sperm cells, thus isolated, can be processed for isolation of nuclear DNA for human identification and forensic DNA analysis.