Method of polymerase chain reaction with ultra-low denaturing temperatures and applications thereof

摘要

The invention relates to a method polymerase chain reaction (PCR) and the application thereof A method of PCR performed at ultra-low denaturing temperatures is provided. The denaturing temperatures of the templates adopted are 93-98° C. in the primary 2-3 cycles, and 60-87° C. in the follow-up cycles, those are much lower than 94-96° C., the conventional denaturing temperatures. It is found in the experiment that this method could not only become a universally applied PCR, but also control the reaction specificity by the template selection at ultra-low temperatures. The method possesses unique functions in excluding non-specific amplified products and false-negative results, excluding false-positivity brought about by the contaminants in products and discriminating genomic DNA from cDNA.