In vivo screening methods for identifying inhibitors of RNA polymerases

摘要

In vivo screening methods for identifying inhibitors of RNA polymerase (RNAP) are provided by the present invention. In certain embodiments, methods according to the invention include steps of: (a) expressing a test RNAP in a cell containing a first reporter; (b) expressing a control RNAP in the cell of step (a) that also contains a second different reporter or, alternatively, a different cell that contains the second different reporter, wherein the first and second different reporters distinguish activities of the test and control RNAPs, respectively; (c) contacting the cell or cells of step (b) with a candidate compound; and (d) assaying to obtain a combined signal from the first reporter and second different reporter wherein a unique combined signal identifies the candidate compound as an inhibitor of an RNAP.