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Method for determining chum salmon haplotype using mitochondrial DNA

机译:利用线粒体DNA确定鲑鲑单倍型的方法

摘要

A kit comprising an oligonucleotide-immobilized substrate obtained by immobilizing, on a substrate, one or more kinds of oligonucleotides that enable detection of a polymorphism in a nucleotide sequence of mitochondrial DNA control region of chum salmon, of which standard is represented by the nucleotide sequence of SEQ ID NO: 8, at a position selected from the 10th, 30th, 42nd, 57th, 70th, 96th, 108th, 154th, 194th, 231st, 242nd, 250th, 260th, 339th, 340th, 386th, 395th, 401st and 471st positions is used to detect the polymorphism based on hybridization of the oligonucleotides with a nucleic acid derived from specimen chum salmon and thereby determine a haplotype of the specimen chum salmon.
机译:一种试剂盒,其包括通过将一种或多种寡核苷酸固定在基质上而获得的固定寡核苷酸的基质,该寡核苷酸能够检测鲑鱼的线粒体DNA控制区域的核苷酸序列中的多态性,其标准由核苷酸序列表示SEQ ID NO:8的第10位,第30位,第42位,第57位,第70位,第96位,第108位,第154位,第194位,第231位,第242位,第250位,第260位,第339位,第340位,第386位,第395位,第395位,第401位和第471位基于寡核苷酸与衍生自标本鲑鱼的核酸的杂交,使用多个位置来检测多态性,从而确定标本鲑鱼的单倍型。

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