Immuno-chromatographic rapid assay in order to detect acid-resistant microorganisms in the stool

摘要

The invention relates to a method for detecting an infection of a mammal with an acid-resistant microorganism comprising the following steps: (a) provision of an immunochromatographic test with a sample application area for the application of a stool sample of the mammal with an antigen and application of the stool sample, (b) incubation of the stool sample using (i) a first receptor under conditions permitting a complex formation of the antigen from the acid resistant microorganism with the receptor; or (ii) at least two different first receptors under conditions permitting a complex formation of the antigen from the acid-resistant microorganism with the at least two first receptors and wherein the first receptor according to (i) or the first receptors according to (ii) specifically bind(s) an antigen which shows, at least with some mammals, a structure after passage through the intestine that corresponds to the native structure or the structure against which a mammal produces antibodies against after being infected or immunized with the acid-resistant microorganism or an extract or lysate thereof or a protein therefrom or a fragment thereof or a synthetic peptide produces antibodies; and (c) provision of a second receptor immobilized at an analysis region, wherein the second receptor binds an antigen receptor complex according to (b) and (d) transport and detection of the formation of at least one antigen receptor complex according to (b) by accumulation of the antigen receptor complex at the second receptor in the analysis area. The invention further relates to an immunochromatographic test which is particularly suitable and designed to carry out the method of the invention.