Cloud-point extraction of enzymes and polypeptides from a fermentation broth using a non-ionic surfactant

摘要

A method of extracting a polypeptide of interest from a fermentation broth comprising:i) adjusting the pH close to pI of the polypeptide of interest;ii) adding a non-ionic surfactant with a hydrophile-lipophile balance (HLB) of 12 or lower;iii) cooling the mixture for solubilization and incubating at above cloud point for extraction;iv) phase separating at below cloud point to obtain liquid-liquid-solid fractions; andv) recovering the surfactant-rich top phase containing the polypeptide of interest.