Primary cells transfected with adenovirus and Methods for Mapping of intracellular Signalling Pathways

摘要

Transfecta cultivation is a primary cell with a Vector comprising a reporter gene operably linked to a CIS element. It induces the expression of a candidate regulatory protein in cell culture, and analyses its effects on the element in CIS.Claim 1: a method for determining whether a Stimulus has the capacity to activate a CIS Regulatory Element in immunocytes in Action, where the action in CIS Regulatory Element is regulated by at least a factor of Transcription Enhancer or where it is known that the stimulus Mule modulates the expression of a way of signalling,The Method includes following steps: (a) a recombinant adenovirus transfecting in immunocytes.Recombinant adenovirus comprising a reporter gene operably linked to the CIS Regulatory Element of Action; (b) measuring a base level of reporter gene activity; (c) to implement the stimulus that immunocytes and (d) to measure the activity of the reporter gene in response to that stimulus.