The production process of biopolu00ecmero type xanthan, biopolu00ecmero obtained, their uses; the culture medium for the growth of Xanthomonas and use thereof for the production of biopolu00ecmero

摘要

"The process of production of xanthan biopolu00ecmero type, biopolu00ecmero obtained, their uses; the culture medium for the growth of Xanthomonas and use thereof for the production of biopolu00ecmero".The present invention relates to a process for the production of biopolymers like xanthan.From lyophilized cultures of bacterial strains isolated from necrotic tissue of hosts with high content of cellulose and hemicellulose in nutritive mediums submergedSubject to the conditions of growth. The strains of the bacteria present productivity of biopolymers from 5.7 to 26.4 grams.Lu00ac - 1u00ac, and these have high viscosity in aqueous solutions with low concentration of biopolymers (0.3 to 4.0%). The values of viscosity are between 100 and 1000 and 4000 MPa. S and 28.000 MPa. S, the deformation rate of 10su00ac - 1u00ac, 1% and 3%, respectively.In the production process, prepares the inoculum in a pre - determined with low concentration of sucrose or glucose.Which is subjected to moderate agitation in temperature of better development of this bacterium (15 to 35 198 C); subsequently,The inoculum is transferred to a liquid medium of fermentation with macro and micro nutrients, B vitamins, vitamin E and / or nicotinamide and high concentration of carbohydrates.This medium is subjected to specific conditions of aeration and agitation, temperature and pH control.Finally, after fermentation to be completed between 24 and 72 hours, is the separation of cells (when required) for centrifuging the fermented medium; subsequently,The biopolymers are insolubilizados, by the addition of salts and polar organic solvents, separated and dried or dried directly when allowed the presence of cells.And pulverized in the particle size desired for use.