a set of u043fu0440u0430u0439u043cu0435u0440u043eu0432 for detection and / or identification of transgenic dna sequences in plant material and containing products (options), primer (wa u0440u0438u0430u043du0442u044b), a pair of u043fu0440u0430u0439u043cu0435u0440u043eu0432 (options)method of detection and / or identification with their use (variants) and a device for implementing the method

摘要

the invention relates to the biotechnology, including genetic engineering, and can be used for monitoring the safety of food, food products and other no interruption of products of mass consumption.the invention u043fu0440u0430u0439u043cu0435u0440u043eu0432 constitute sets.a pair of u043fu0440u0430u0439u043cu0435u0440u043eu0432 and individual u043fu0440u0430u0439u043cu0435u0440u044b for detection and / or identification of transgenic dna sequences in plant material and containing productsmethods of detection and / or identification with their use and device for the implementation of one of the ways.u043fu0440u0430u0439u043cu0435u0440u044b u043au043eu043cu043fu043bu0435u043cu0435u043du0442u0430u0440u043du044b 5 typical u043cu0430u0440u043au0435u0440u043du044bu043c genes and regulatory dna sequences of the gene gus bacteria e.coli, the gene npt ii of u0442u0440u0430u043du0441u043fu043eu0437u043eu043du0430 Tn5, 35S - u043fu0440u043eu043cu043eu0442u043eu0440u043du043eu043cu0443 the cauliflower mosaic virus, u0442u0435u0440u043cu0438u043du0430u0442u043eu0440u043du044bu043c site-specific gene and the gene of the ocs a.tumefaciens most often contained in the genetic constructions of the most popular in the world market of transgenic plants, and have the nucleotide u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u0438,the SEQ id nos. 1 to 10.the original selection of u043fu0440u0430u0439u043cu0435u0440u043eu0432 and introduction in their sequence similarity and their nucleotide substitutions provide correction of thermodynamic parameters and paul their compatibility in sets.that allows them to work effectively in the same reaction conditions, and greatly increases the specificity.the sensitivity and the accuracy of the methods of detection and / or identification of transgenic dna sequences with their participation.methods include dna extractions from plant material and containing products, the asymmetrical or symmetrical pcr, including u043cu0443u043bu044cu0442u0438u043fu043bu0435u043au0441u043du043eu0439,with the u044du043au0441u0442u0440u0430u0433u0438u0440u043eu0432u0430u043du043du043eu0439 dna and a set of u043fu0440u0430u0439u043cu0435u0440u043eu0432,division of u0430u043cu043fu043bu0438u0444u0438u0446u0438u0440u043eu0432u0430u043du043du044bu0445 products react with their subsequent u0434u0435u0442u0435u043au0446u0438u0435u0439 and / or identification by hybridization with u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044bu043cu0438 biological icri u043eu0447u0438u043fu0435 u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u0430u043cu0438 or method of gelelectrophoresis, or with the assistance of u0430u043cu043fu043bu0438u0444u0438u043au0430u0442u043eu0440u0430 in real-time.the use of the method in the analysis of the results of pcr u0433u0438u0431u0440u0438u0434u0438u0437u0430u0446u0438u043eu043du043du043eu043c specially developed biological microarrays mb "u0442u0440u044du0441u0441u0438u0433u0435u043du00bb - u0430u043cu0438u043du0438u0440u043eu0432u0430u043du043du044bu0445 glasses with u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044bu043cu0438 directly on themthe surface u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u0430u043cu0438 with u043du0443u043au043bu0435u043eu0442u0438u0434u043du044bu043cu0438 sequences specified in the SEQ id nos.11 - 15, and also the hardware and software of the u0434u0435u0433u043cu0438u0433u0435u043du00bb significantly increases the specificity, efficiency and technology process.