IN VITRO METHOD FOR THE DIAGNOSIS OF CARDIOVASCULAR FUNCTIONALITY OF BONE MARROW-PRECURSOR CELLS (BMPs) AND/OR BLOOD DERIVED CIRCULATING PRECURSOR CELLS (BDPS)

摘要

The invention relates to an in vitro method for the analysis of a sample extracted from a mammal in relation to cardiovascular diseases. Said method comprises the following steps: a) the bone marrow precursor cells (BMP) and/or circulation precursor cells (BDP) derived from blood (BDP) are insulated by means of cell specific surface markers, and b) the cardiovascular functionality of the insulated BMP and/or BDP is examined by means of a suitable migration test. The inventive method can be used in the form of a kit in the field of diagnosis and/or prognosis of cardiovascular diseases, can be used to monitor the therapy thereof and/or for the stratification of cell therapy planted with stem cells and progenitor cells in order to increase perfusion of ischaemic tissue and/or for the regeneration of tissue loss (e.g. heart failure). The invention also relates to an in vitro method for the insulation of specific bone marrow precursor cells by means of a suitable migration test. Said BMP and/or BDP can be used in the treatment of cardiovascular diseases, selected from the group comprising stable coronary diseases, acute coronary syndrome, acute myocardial infarction, chronic ischaemic cardiomyopathy (ICMP), dilatative cardiomyopathy (DCM) or other causes of cardiac insufficiency.