A Simple process for separation and purification of nucleic acids directly from cell lysates using reversibly soluble smart polymers

摘要

a) binding of polymer to nucleic acids from the cell lysate to form a polymer-nucleic acid complex, b) separation of the polymer-nucleic acid complex, c) dissolution of the complex, d) removal of polymer from dissolved complex, under various specified conditions to obtain pure nucleic acid of interest in active form that can be useful for various molecular biological applications.