A PATHOGEN DETECTION SYSTEM AND METHOD FOR PATHOGEN ANALYSIS .

摘要

Abstract:The invention discloses methods and apparatus for implementing microfluidic analysis devices areprovided. A monolithic elastomer membrane associated with an integrated pneumatic manifoldallows the placement and actuation of dense arrays of a variety of fluid control structures, such asstructures for isolating, routing, merging, splitting, and storing volumes of fluid. The fluid controlstructures can be used to implement a pathogen detection analysis system including integratedimmunoaffinity capture and analysis, such as polymerase chain reaction (PCR) and capillaryelectrophoresis (CE) analysis. An analyte solution can be input into the device and pumped through aseries of immunoaffinity capture matrices in microfabricated chambers having antibodies targeted tothe various classes of microbiological organisms such as bacteria, viruses and bacterial spores. Theimmunoaffinity chambers can capture, purify, and concentrate the target for further analysis steps.