Process for preparing monoclonal antibodies; hybridoma cell lines generated by this process and recombinant chimeric virus-like particles with inserted foreign protein fragments as immunogenes to generate hybridomas by this process

摘要

The present invention relates to the process for preparing monoclonal antibodies based on recombinant chimeric viral proteins which proteins are able to form virus-like particles. Viral proteins mentioned are used as carriers for peptides and epitopes against which the monoclonal antibodies are to be obtained. The chimeric viral proteins claimed have fragments of other proteins (epitopes) incorporated in appropriate places which fragments are exposed onto the virus-like particles surfaces. The immunogenity of chimeric proteins mentioned above is higher than that of earlier known immunogens and they promote formation of monoclonic antibodies more effectively. The present invention relates to biotechnology and may be used in hybridoma technique for preparing monoclonal antibodies against desirable epitopes. The present invention also presents hybridomes obtained by this process.