Elimination of bovine spongiform enzephalopathy from excrement of cattle or the excrement produced substances and excrement liquid, comprises detecting the substance similar to neopterin by high speed liquid chromatography

摘要

The elimination of bovine spongiform enzephalopathy from excrement of cattle or the excrement produced substances and excrement liquid, comprises detecting the substances similar to neopterin by high-speed liquid chromatography (HPLC). The excrement liquid is obtained by a centrifugation of the diluted excrement of the cattle and is separated from impurities by a cellulose membrane in 3 ml water. A chromatogram is created with a fluorescence detector with a detection wavelength of 438 Nm and an excitation wavelength of 353 Nm. The elimination of bovine spongiform enzephalopathy from excrement of cattle or the excrement produced substances and excrement liquid, comprises detecting the substances similar to neopterin by high-speed liquid chromatography (HPLC). The excrement liquid is obtained by the centrifugation of the diluted excrement of the cattle and is separated from impurities by a cellulose membrane in 3 ml water. A chromatogram is generated with a fluorescence detector with a detection wavelength of 438 Nm and an excitation wavelength of 353 Nm. The obtained liquid (0.01 ml) is evaporated and dissolved with 0.1 ml of mixture from 0.1 mol/l NaOH and 0.1 mol/l iodine in Al, is separated from insoluble material by the centrifugation, is left at ambient temperature for 1 hour, and is injected into the HPLC device. The detection is carried out by radioimmunoassay (RIA) or radioactive enzyme-linked immunosorbent assay (ELISA). The excrement of the cattle is centrifuged for 10 minutes for the production of excrement liquid, which is frozen. The excrement fluid with 0.05 ml is mixed with a marked anti-neopterin anti-bodies substance (0.1 ml). Six standards of neopterin are used for the detection of the substance. The marker substance is a solution from iodine-125-neopterin. The produced mixtures are left for one hour, are mixed with 1 ml per washing solution, and centrifuged for 10 minutes. The radioactivity of each individual mixture is measured for 1 minute, by a gamma counter. A mass spectroscopy, and a light absorption in the ultraviolet, visible, infrared or Roentgen region are used as the detection procedure.