Biochip and device for reading a biochip comprising a plurality of molecular recognition areas.

摘要

Reading device in real time of a biochip comprising a plurality of zones (110) of recognition and a plurality of marks (122, 124, 124a, 221, 222, 223, 225, 227) optical positioning, comprising areas recognition fragments of DNA or RNA and occupying, with respect to the optical positioning marks, predetermined locations, the device comprising: - a head (20) adapted to project a light incident on the biochip optical, - means (14 and / or 24 ) relative displacement of the head and said biochip, capable to sweep the biochip, and comprising means (14) macroscopic displacement (large scale) and means (24) microscopic displacement (small scale), - a first system (40) optical called analysis system associated with said optical head to project on at least one first detector (42) electrooptical a susceptible light proceeding from recognition areas, - a second system (60) optical called positioning system associated with the optical head to project on at least one second detector (62) electrooptical a susceptible light proceeding from at least one positioning mark, and - servomechanisms (70) means moving said optical head to control the moving means according to electrical signals from the electro-optical detector system (62) optical positioning servomechanisms (70) being connected to the means (24) microscopic displacement, wherein the optical head (20) comprises a lens (22) for focusing and at least one actuator (24) axial and / or lateral focusing lens (22), and comprising a third system (80) optical called focus, associated with the optical head (20), to project onto a third electrooptical sensor a light from the reflection of incident light on the biochip, and servomechanisms (70) approach associated with the actuator (24) for controlling the displacement of the lens focus.