SYSTEMS AND METHODS FOR PROTEIN PRODUCTION EMPLOYING POLYPEPTIDES FUNCTIONAL IN UNFOLDED PROTEIN RESPONSE PATHWAY

摘要

The invention relates to systems and methods for producing proteins ofinterest. The invention employs genetically-engineered animal or plant cellsthat have modified protein folding or processing capacities. In one aspect,the invention features genetically-engineered cells comprising one or morerecombinant expression cassettes which encode (1) a protein of interest and(2) a polypeptide that is functional in the unfolded protein response (UPR)pathway of the cells. Co-expression of the polypeptide significantly increasesthe yield of the protein of interest in the genetically-engineered cells. Inone example, the genetically-engineered cells aare animal cells, and the co-expressed polypeptide is a component or modulator of an XBP1- or ATF6-mediated UPR pathway.