RAPID AND EFFICIENT CDNA LIBRARY SCREENING BY SELF-LIGATION OF INVERSE PCR PRODUCTS

摘要

The invention provides a method for screening, isolation and recovery of clones using self-ligation of inverse PCR products. The recovery of full-length, intact clones representing genes and alternatively spliced transcripts of interest is described. We demonstrate the utility of the method by recovering full-length cDNA clones for genes and alternatively spliced transcripts, including genes that are not represented in available EST collections. The method is applicable to any plasmid library, including genomic libraries.