ITERATIVE PROBE DESIGN AND DETAILED EXPRESSION PROFILING WITH FLEXIBLE IN-SITU SYNTHESIS ARRAYS

摘要

The present invention provides for detecting and reporting the method and composition of multiple and different subject polynucleotide sequences in sample, such as the polynucleotides corresponding to the multiple and different genes expressed by cell or cell. Particularly, the present invention provides the methods for screening multiple candidate polynucleotide probes, to assess the sensibility and specificity that each candidate probe hybridizes with subject polynucleotide sequence. Then they can be ranked up according to the sensibility and specificity of candidate polynucleotide probe, and can choose the probe that there is best sensibility and specificity to subject polynucleotide sequence. In one embodiment, it can be according to method choice polynucleotide probes described herein to prepare " screening chip ", wherein detecting a large amount of subject polynucleotide sequences using single microarray has several (for example, 1-5) a probes for being directed to each subject polynucleotide sequence. In a particularly preferred embodiment, the present invention provides a kind of screening chip, which is able to detect the genetic transcription from organism whole gene group. In an alternative embodiment, can according to method choice polynucleotide probes described herein, with preparation " feature chip ", with use several polynucleotide probes (such as: 10-20) each signature gene. The present invention also provides include the microarray for cell or the polynucleotide probes of the lots of genes of organism expressing. In addition, the method for detecting multiple polynucleotide molecules is additionally provided, including the lots of genes by cell or organism expressing.