首页>
外国专利>
SURFACE ASSEMBLY FOR IMMOBILIZING DNA CAPTURE PROBES IN GENETIC ASSAYS USING ENZYMATIC REACTIONS TO GENERATE SIGNAL IN OPTICAL BIO-DISCS AND METHODS RELATING THERETO
SURFACE ASSEMBLY FOR IMMOBILIZING DNA CAPTURE PROBES IN GENETIC ASSAYS USING ENZYMATIC REACTIONS TO GENERATE SIGNAL IN OPTICAL BIO-DISCS AND METHODS RELATING THERETO
展开▼
机译:利用酶反应在光学生物盘片中产生信号的用于在遗传学分析中固定DNA捕获问题的表面组装及其方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates to methods and systems for the detection of specific sequences of nucleic acids or oligonucleotides. It relates more particularly to a conjugated enzyme based assay system utilizing reflective and/or transmissive optical bio-discs for detection of specific sequences of nucleic acids. In the assay according to the present invention, an enzyme reaction is used to detect the presence of an analyte (DNA or RNA) in a microchannel in an optical bio-disc. The analyte is immobilized by hybridization with a specific capture probe on a capture layer on the surface and the signal that is generated is localized and specific. The signal can be in the form of a pellet, a fluorescent product, and/or a colored product, and can be detected and quantified by an optical bio-disc reader utilized in conjunction with the inventions hereof. This assay is thus quantitative in nature.
展开▼