TARGET ENRICHMENT METHOD OF STEM CELLS AND MAGNETIC ENRICHMENT DEVICE FOR TARGET LOCALIZATION

摘要

The present invention discloses a target enrichment method of stem cells and a magnetic enrichment device for target localization. The target enrichment of stem cells said in the invention includes the following steps: 1) labeling stem cells with immunomagnetic beads of 30-70nm to obtain labeled stem cells; 2) injecting labeled stem cells into circulatory system of target tissue; 3) locating the body surface region which corresponds to the target tissue to face two mutually attracting magnetic poles, and enriching said stem cells into target tissue during the circulation of circulatory system. The magnetic intensity of said two mutually attracting magnetic poles is 9200-24500Gs. According to the present invention, the stem cells are labeled with immunomagnetic beads, and the magnetic enrichment device for target localization is used to create high intensity and gradient magnetic field by utilizing skillfully magnetic nature of superparamagnetic beads in order to enrich given cells into target tissue. The invention has the advantages of high enrichment efficiency, convenience and so on and it is applied broadly.