METHODS FOR THE STEREOSELECTIVE SYNTHESIS AND ENANTIOMERIC ENRICHMENT OF B-AMINO ACIDS

摘要

The present invention relates to methods for the stereospecific synthesis and for the enantiomeric enrichment of Beta-amino acids. A novel D-Beta-aminotransferase, which exhibits stereoselectivity for D-Beta phenyalanine D-3-amino-3-phenylpropanoic acid, was purified from a newly-isolated strain of Variovorax paradoxus. A novel L-Beta- aminotransferase was purified from a newly-isolated strain of Alcaligenes eutrophus. The D- and L-Beta-aminotransferases can be used to facilitate the stereoselective biosynthesis of Beta-D-phenylalanine or Beta-L-phenylalanine, from a mixture of L-glutamic acid or L-alanine, respectively, and 3-keto-3-phenypropanoic acid in the presence of the cofactor pyridoxal photephate.