THE PREPARATION METHOD OF HALICYLINDRAMIDE A AND C FROM A MARINE SPONGE(HALICHONDRIA SP.), AND ANTIFUNGAL AND ANTIBACTERIAL APPLICATION THEREOF

摘要

The present invention relates to a novel separation method of Halicylindramide A and C extracted from sponges Halichondria sp. And antifungal and antibacterial agents using the same. To extract this material, the sponges halychondria are lyophilized to completely remove moisture, extracted with dichloromethane and methanol, and the solvent is evaporated under reduced pressure. The solvent is removed under reduced pressure from the methanol layer obtained by partition extraction of the residue into methanol and n-hexane. The residue is partitioned and extracted again with ethyl acetate and water, and the obtained water layer is partitioned and extracted with n-butanol. The solvent of the n-butanol layer thus obtained is removed under reduced pressure, and the residue is purified by resin column chromatography and reverse phase semi-preparative HPLC to obtain the above substances. The halycillin dramides A and C thus obtained have excellent inhibitory activity against various fungal and bacterial species and exhibit low cytotoxicity.