A PROCESS OF PREPARING DENDRITIC CELLS FROM BLOOD CORPUSCLES OF PATIENT SUBJECTED TO MALIGNANT TUMOR, AND A PHARMACEUTICAL COMPOSITIONS SAID DENDRITIC CELLS AS EFFECTIVE INGREDIENT

摘要

The present invention provides a method of mobilizing granulocyte colony stimulating factor (G-CSF) to a patient with a malignant tumor for peripheral blood flow of peripheral blood stem cells produced in the patient's bone marrow. Thereby promoting the hematopoietic function of the bone marrow; (b) collecting and isolating blood corpuscles containing hematopoietic stem cells from the peripheral blood; And (c) fibromyalgia syndrome related tyrosine kinase 3-ligand (Flt3-ligand), granulocyte-macrophage colony stimulating factor (GM-CSF), interleukin-4 (IL-4) and tumor necrosis factor (TNF-α) A method for producing dendritic cells comprising the step of inducing differentiation of the isolated blood cells into dendritic cells in vitro using a cytokine cocktail comprising To dendritic cells. The present invention also relates to a pharmaceutical composition for treating or preventing malignant tumors comprising the dendritic cells and a pharmaceutically acceptable carrier or excipient.;In the present invention, cytokine cocktail including Flt3-ligand, GM-CSF (CSF2), IL-4 and TNF-α for all without separating peripheral blood cells into CD34 ⁺ cells, monocytes, neutrophils, etc. Can be used to induce differentiation into dendritic cells.