Novel rhodococcus, rhodococcus-origin nitrilase gene, nitrilehydratase gene and amidase gene and process for producing carboxylic acids by using the same

摘要

By hydrolyzing the cyano group of a nitrile compound by using also a novel Rhodococcus bacterium Lactococcus and method of producing carboxylic acid. Excellent location for the cyano group of aromatic polynitrile compounds exhibit selectivity Rhodococcus - nitrilase gene derived from, nitrile hydratase gene, and the amino is transformed transformants by plasmids containing a gene used in a method for producing carboxylic acids (specifically, cyano carboxylic acids); These transformants, plasmids, and production method of the enzyme gene by using the transformant; And the thus obtained enzyme. The thus obtained carboxylic acids (specifically, cyano carboxylic acids) are useful as starting materials in drugs, pesticides, dyes and other synthetic chemicals.