-3 Method for screening activity-regulator of Human PRL-3 protein using nuclear magnetic resonance spectrometer

摘要

Method using a nuclear magnetic resonance spectrometer for screening active modulators of human PRL-3 protein, a device and such human PRL-3 protein fragments for screening methods therefor are disclosed. The screening methods are amino acid residues are labeled with isotopes 15N or 13C, to prepare the protein samples on the solution containing the active domain of human PRL-3 protein; Step of analysis by using a nuclear magnetic resonance spectroscopy on a protein sample solution, determining the structure of the protein sample in a solution state; And the addition of human PRL-3 protein activity inhibiting candidates to a solution containing the protein samples and by analyzing the protein samples using nuclear magnetic resonance spectroscopy, if the active control agent or the substrate binding to the protein appearing peak pattern and a step to determine whether the occurrence. ; Nuclear magnetic resonance spectroscopy, the human PRL-3, a protein, an active agent, a substrate, a peak pattern