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A PROCESS FOR THE PURIFICATION OF CLOSTRIDIUM BOTULINUM NEUROTOXIN TYPE B USING IMMNUNO-AFFINITY CHROMATOGRAPHY
A PROCESS FOR THE PURIFICATION OF CLOSTRIDIUM BOTULINUM NEUROTOXIN TYPE B USING IMMNUNO-AFFINITY CHROMATOGRAPHY
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机译:免疫亲和层析法纯化肉毒梭菌神经毒素B型的工艺
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摘要
The invention (a) by coupling an antibody specific for Clostridium botulinum type B toxin to a support to prepare an antibody binds a support; (B) dissolving the precipitate obtained by acidifying the culture medium of the strain Clostridium botulinum type B in the pH 3~4 buffer solution; And (c) (b) the solution obtained, and step (a) Claus tree purification of Stadium botulinum type B toxin comprising the step of performing the antibody affinity column chromatography produced an antibody using the combined support in It provides. ; Clostridium botulinum, toxin type B, antibody affinity chromatography
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