ACADM DNA DNA Detection of DNA mutation in the porcine ACADM promoter region and their application as DNA markers for the early selection of pigs having high intramuscular fat content

摘要

invention ACADM (acyl-coA dehydrogenase, medium-chain) gene polymorphism analysis and DNA from the resulting relates to the correlation analysis of intramuscular fat of pigs to help utilize it , intramuscular fat, high pig is about the development of DNA markers that can be selected early . ; the present invention , pigs regulation of gene ACADM nucleotide mutation sites were found at the site , this site by making a primer for amplification to amplify and then was subjected to PCR-RFLP fraud . ; the object and intramuscular fat, the genotype of the measured association analysis , was found to exhibit a high correlation with intramuscular fat, genotype , which can be used as a test method that can be selected in early pig intramuscular fat, which is expected to be high .