METHOD FOR PRODUCING ANTIBACTERIAL and antioxidant fraction from the buckthorn seed (HIPPOPHAE RHAMNOIDES L.)

摘要

1. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 u0430u043du0442u0438u0431u0430u043au0442u0435u0440u0438u0430u043bu044cu043du043eu0439 factions of the innovative parts u043eu0431u043bu0435u043fu0438u0445u0438 (latvian private company rhamnoides l.) for the stage;(i) which u0441u0435u043cu00a0u043d u043eu0431u043bu0435u043fu0438u0445u0438 (latvian private company rhamnoides l.) water u0434u043bu00a0 u0443u0434u0430u043bu0435u043du0438u00a0 any u0437u0435u043cu043bu00a0u043du043eu0433u043e and outside material, followed by drying.;(ii) the use u0441u0435u043cu00a0u043d dried in powder and particle size of 60 to 80 u043cu0435u0448.,;(iii) extractions of dried u0441u0435u043cu00a0u043d u0440u0430u0441u0442u0432u043eu0440u0438u0442u0435u043bu00a0u043cu0438 u043bu0435u043eu0442u0440u043eu043fu043du043eu0439 series in u043fu043eu0440u00a0u0434u043au0435 u0443u0432u0435u043bu0438u0447u0435u043du0438u00a0 effect u044du043bu044eu0438u0440u043eu0432u0430u043du0438u00a0 with subsequent least read several others methanol. the teachings of;(iv) filtering the extract obtained u0434u043bu00a0 u0443u0434u0430u043bu0435u043du0438u00a0 any particles and u043fu043eu043bu0443u0447u0435u043du0438u00a0 extract not containing particles;(v) u0434u0438u0441u0442u0438u043bu043bu00a0u0446u0438u044e extract not containing particles u0434u043bu00a0 regeneration of solvent;(vi) the concentration of u0434u0438u0441u0442u0438u043bu043bu0438u0440u043eu0432u0430u043du043du043eu0433u043e extract not containing particles in a vacuum to obtain crude u043cu0435u0442u0430u043du043eu043bu044cu043du043eu0433u043e extract.;(vii) drying a concentrated crude extract with an extract from u0430u043du0442u0438u0431u0430u043au0442u0435u0440u0438u0430u043bu044cu043du043eu0439 and / or u0430u043du0442u0438u043eu043au0438u0441u043bu0438u0442u0435u043bu044cu043du043eu0439 activity.;2. method for 1, in which the seeds u043eu0431u043bu0435u043fu0438u0445u0438 washed running water and then dried at 40 - between for 3 to 4 hours.;3. method for 1, in which the extractions of u0441u0435u043cu00a0u043d u043fu0440u043eu0432u043eu0434u00a0u0442 in the extractor u0441u043eu043au0441u043bu0435u0442u0430 at a temperature of 55 60u0441 for 8 to 9 hours.;4. method for 1, in which the filtrate extract u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 using filter paper Whatman no. 41.;5. method for 1, in which u043fu0435u0440u0435u0433u043eu043du043au0443 u043fu0440u043eu0432u043eu0434u00a0u0442 in u0434u0438u0441u0442u0438u043bu043bu00a0u0446u0438u043eu043du043du043eu0439 installation with 60 - ranging on the temperature u043au0438u043fu0435u043du0438u00a0 u0434u043bu00a0 regeneration of 80 - 90% of u0440u0430u0441u0442u0432u043eu0440u0438u0442u0435u043bu00a0.;6. method for u0440u0435u0446u0438u0440u043au0443u043bu0438u0440u0443u044eu0442 p.5, which u0440u0435u0433u0435u043du0435u0440u0438u0440u043eu0432u0430u043du043du044bu0439 solvent in extractor u0441u043eu043au0441u043bu0435u0442u0430 u0434u043bu00a0 u043fu0440u043eu0432u0435u0434u0435u043du0438u00a0 further extraction.;7. method for 1, in which the extract, containing particles, are concentrating in a vacuum at a pressure of 200 - 250 mbar and evaporator temperature 45 between receipt of raw u043cu0435u0442u0430u043du043eu043bu044cu043d wow extract.;8. method for 1, in which a drying concentrated crude extract u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 u0432u044bu043cu043eu0440u0430u0436u0438u0432u0430u043du0438u0435u043c gaining faction with the u0430u043du0442u0438u043eu043au0438u0441u043bu0438u0442u0435u043bu044cu043du043eu0439 activity.;9. method for 1, in which a drying concentrated crude extract u043fu0440u043eu0432u043eu0434u00a0u0442 vacuum furnace at a pressure of 175 - 200 mbar and 40 between during 8 - 9 hours to obtain the faction has the next u0430u043du0442u0438u0431u0430u043au0442u0435u0440u0438u0430u043bu044cu043du043eu0439 activity.