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METHOD FOR PHOTOMETRIC DETECTION OF URINARY IODINE
METHOD FOR PHOTOMETRIC DETECTION OF URINARY IODINE
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机译:光度法测定尿中碘的方法
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摘要
FIELD: biochemistry.;SUBSTANCE: the present innovation deals with laboratory techniques of investigation and includes centrifuging and evaporating a sample, transferring iodine into iodate-ions followed by treatment with a color reagent and measuring optic density of the solution obtained. Before centrifuging organic compounds in urinary sample should be transferred into residue, centrifugate should be supplemented with chloroform; afterwards the inferior chloroformic layer should be treated with chlorine water, aqueous phase should be separated to remove chlorine residues. Then one should add 1 n potassium iodide solution and 0.1 n sulfuric acid solution followed by photometering the solution at wave length being 400 nm. Organic compounds in a sample should be precipitated due to adding barium hydroxide and zinc sulfate.;EFFECT: higher sensitivity and accuracy of detection.;1 cl, 2 tbl
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