METHOD FOR HEPATIC FIXATION AT NARCOTIZED ANIMAL'S LIFE PERIOD

摘要

FIELD: experimental medicine.;SUBSTANCE: the present innovation deals with preparing hepatic preparations for histological testing. In the course of an experiment on inducing hepatic pathology animals should be perfused with glutaraldehyde formaldehyde fixing agent through portal vein under conditions of narcosis. Then comes perfusion with incubation medium containing 0.2%-digitonin in Flickinger's solution at pH being 7.2 at 20-22°C. After incubation hepatic tissue should be reduced to be placed into 0.2%-digitonin for 4-10 h, and then samples should be washed with 0.1 M cacodylate buffer and postfixed with 1%-solution upon cacodylate buffer for 2 h. Then it is necessary to conduct dehydration to be put into araldite followed by contrasting with uranylacetate and lead citrate. The method enables to increase degree of contrast due to fixing hepatic tissues in situ and optimal matching incubation medium.;EFFECT: higher efficiency.;3 ex