RECOMBINANT PLASMIDE ENCODING HYBRID GST-ESAT-6 POLYPEPTIDE HAVING ACTIVITIES OF SPECIES-SPECIFIC MYCOBACTERIAL ESAT-6 ANTIGEN, RECOMBINANT ESCHERICHIA COLI STRAIN AS PRODUCER OF HYBRID GST-ESAT-6 POLYPEPTIDE AND RECOMBINANT GST-ESAT-6 POLYPEPTIDE

摘要

FIELD: biotechnology and gene engineering.;SUBSTANCE: invention relates to recombinant plasmide encoding hybrid GST-ESAT-6 polypeptide having activities of species-specific mycobacterial ESAT-6 antigen. Plasmide has molecular mass of 3.45 MD and size of 5315 n.p. Recombinant E.coli polypeptide containing such plasmide and recombinant GST-ESAT-6 polypeptide also are disclosed. Recombinant protein has activities of species-specific antigen protein Mycobacterium tuberculosis ESAT-6. Method of present invention makes in possible to simplify purification process of recombinant polypeptide and to produce protein of high purity hawing activities of mycobacterial ESAT-6 antigen without degradation thereof.;EFFECT: earlier species-specific diagnosis of tuberculosis infection.;3 cl, 3 dwg, 4 tbl, 5 ex