STRAIN OF BACTERIUM Glacial ice bacterium I AS PRODUCER OF RESTRICTION ENDONUCLEASE Gla I

摘要

FIELD: biotechnology, genetic engineering, biochemistry, enzymes.;SUBSTANCE: invention relates to preparing a novel strain of microorganism used for isolation of the novel restriction endonuclease Gla I. The strain Glacial ice bacterium I is isolated from a reservoir as result of the search of producers of restrictases and provides preparing the restriction endonuclease Gla I recognizing and cleaving both chains of DNA nucleotide sequence comprising C5-methylcytosine bases in the recognition site 5'-GCGC-3'. Invention provides preparing the yield of enzyme 10 U/g of crude biomass with specific activity of the end enzyme 200 U/ml. This novel restriction endonuclease can be used for detection of methylated DNA.;EFFECT: valuable biological and biochemical properties of enzyme.;1 tbl, 3 dwg, 4 ex