A method for the production of dna - probes for dna - sections with repetitive sequences

摘要

A method for the production of dna - probes with single strand dna - - oligomers of a repetitive sequence motif,characterized byProviding the single strand - dna - oligomers of the repetitive sequence motif as abnormal,Adding to the abnormal complementary oligomers as against sense strand,Can hybridize of sense - and to sense strand,Adding a first primer, consisting of a first primer sequence and oligomers of the repetitive sequence motif, wherein the first primer sequence is not connected to the repetitive sequence motif is identical, and adding a second primer consisting of a second primer sequence and the to the abnormal complementary oligomers of the repetitive sequence motif, wherein the second primer sequence is not connected to the repetitive sequence motif is identical,The primers hybridize,The addition of nucleotides, of a klenow - fragment and a ligase and allowed to react,Removal of single-stranded dna and dna with a length less than 100 nucleotides,Amplification of the purified dna by means of pcr andMarking of the pcr - products with a reporter molecule.