cloning and production of n. i bst / political nicking endonuclease and related methods for the use of nicking endonucleasen in the single stranded verdru00e4ngungsamplifikation

摘要

The invention provides a method of making a mutated Type IIT endonuclease which has nicking activity comprising the steps of: (a) identifying a heterodimeric Type IIT endonuclease; (b) identifying a conserved region within said Type IIT endonuclease; (c) generating at least one mutation within said conserved region; and (d) analyzing the mutant endonuclease of step (c) for nicking endonuclease activity.