process for the production of hepatitis b surface antigen recombinantem with enhanced immunogenic capacity and use in a impfstoffzubereitung

摘要

A multi-step process for recovering hepatitis B surface antigen from Pichia pastoris cells containing a gene encoding said hepatitis B surface antigen and having expressed it. The process leads to a high yield of very pure, homogeneous HBsAg in a particulated form having a high immunogenicity, suitable for use in vaccine compositions for treating human beings and for use in diagnostic methods.