METHOD FOR DETERMINING SEX, REAGENT FOR DETERMINING SEX, METHOD FOR DETERMINING RETROGRADE EMBRYO AND REAGENT FOR DETERMINING RETROGRADE EMBRYO

摘要

PROBLEM TO BE SOLVED: To provide a method for determining sex, with which the sex of an embryo having reached a blastocyst is easily determined in a noninvasive manner; to obtain a reagent for determining sex; to provide a method for determining a retrograde embryo, with which whether an embryo having reached a blastocyst retrogrades or not is determined in early stages; and to obtain a reagent for determining a retrograde embryo.;SOLUTION: An embryo having reached a mammalian blastocyst is cultured in a culture solution containing fluorescent pigment having active oxygen sensitivity under a fixed culture condition and then a first fluorescence image is obtained. The embryo is cultured in a culture solution containing fluorescent pigment and a glucose having a fixed concentration under a fixed culture condition and then a second fluorescence image is obtained. The first fluorescence image is compared with the second fluorescence image to determine the sex of the embryo. An embryo having reached a mammalian blastocyst is cultured in a culture solution containing fluorescent pigment having active oxygen sensitivity under a fixed culture condition and then a fluorescence image is collected. The fluorescence intensity of the fluorescence image is obtained; the fluorescence intensity is compared with a preset threshold value to determine whether a retrograde action occurs or not in the embryo.;COPYRIGHT: (C)2007,JPO&INPIT