Use of the modular endonuclease in order to produce new modular II type restriction endonuclease CspCI, and the endonuclease which possesses new peculiarity

摘要

The new restriction endonuclease and its production method rearrange shitorobakuta (Citrobacter) kind 2144 (NEB#1398) or and the stocks colon bacillus (Escherichia coli) NEB#1554 (this cuts off) from is available nt arrangement 5 inside two these chain DNA molecules ' -caannnnngtgg-3' (arrangement number 14) in. As for the said new restriction endonuclease, the peculiarity part, is the module which becomes independent from restriction - decoration module, it is the modular protein.