And methods for analysis platform for the creation of protein expression profiles of the cell population

摘要

Qualitative and cell populations, including the following quantitative protein expression profiles and / or, in particular, to a method which is performed by using the same and analytical platform for creating a differential protein expression profiles present invention: To create a lysate of the cell population of more than -1, solubilized solution contains multiple proteins expressed by the respective cell population, - Providing a solid support in a substantially planar, - Or, directly on the solid support in undiluted form or diluted, the adhesion promoting layer, which is applied to the solid support, deposited on separate sites Cell lysates small amount, thereby You can create a two-dimensional array one-dimensional or one or more of the measurement separate areas on the solid support, - For example, or global analysis of signal transduction pathways, the specificity of the highest selectivity and for the screening of antibody set / library against protein targets for affinity, it is included in the cell lysate in the measurement area of ​​the separate to apply the binding of multiple reagents as specific binding partners of the protein to be detected, and if appropriate, to apply a detection reagent in one or more arrays of measurement areas of the one or more, the and the binding reagents After the detection reagent, or is applied sequentially bound to the binding reagent in the measurement area of ​​the discrete array of one or more detection reagents, and is applied in a single addition step, - Can be measured and recorded in a manner that was locally resolved the light signal resulting from the measurement area separate array of the one or more, Solid support planar adhesion promoting layer and a non-porous, it may be applied is less than 1μm thick said substantially here.