RNA APTAMER INHIBITING TERMINATION FACTOR OF ESCHERICHIA COLI AND ITS UTILIZATION

摘要

PPROBLEM TO BE SOLVED: To provide an RNA aptamer specifically inhibiting a termination factor of Escherichia coli. PSOLUTION: The RNA aptamer has an inhibiting activity to termination factor (RF1) derived from Escherichia coli. PCR proliferation is carried out by using a part of a T-7 promoter sequence and random sequence as a template. Then, the proliferated DNA pool is used as a template and RNA library is prepared by using T-7RNA polymerase. Further, the RNA aptamer is selected by carrying out binding selection test to a recombinant Escherichia coli RF1. PCOPYRIGHT: (C)2007,JPO&INPIT