Method for producing a stable A549 cell lines in serum-free medium suspension culture

摘要

The present invention provides a method for adapt (eg, A549 cells), are grown in non-animal material-free medium suspension culture and serum-free. The present invention provides a method for preparing (eg, adenovirus) viruses are the A549 cells derived from the adapted for growth in non-animal material-free medium suspension culture and serum-free. The adapted A549 cell line, has the characteristics of cell lines that are identified as (ATCC) registration number No. PTA-5708 Issue American Type Culture Collection.