pH DEPENDENT ION EXCHANGE MATERIAL HAVING SELECTIVELY HIGHER BINDING FORCE TO NUCLEIC ACID THAN TO PROTEIN, SOLID SUBSTRATE WITH ION EXCHANGE MATERIAL IMMOBILIZED THEREON, AND METHOD FOR ISOLATING NUCLEIC ACID UTILIZING MATERIAL AND SOLID SUBSTRATE

摘要

PPROBLEM TO BE SOLVED: To provide a pH dependent ion exchange material having selectively higher binding force to nucleic acids than to proteins, a solid substrate with the ion exchange material immobilized thereon, and a method for isolating a nucleic acid using the material and the solid substrate. PSOLUTION: This pH dependent ion exchange material comprises a constitutional unit derived from at least one monomer selected from the group consisting of chemical formulae MO, M1, M2, and M3 and at least one constitutional unit derived from A-containing monomer and B-containing monomer and has a degree of polymerization of 2 to 30,000. In the formulae, A represents -X(CHSB2/SB)nY, wherein n is an integer of 1 to 10, X represents a functional group reactive with an activated ester and Y represents an aromatic heterocyclic base containing a primary, secondary or tertiary amino group or nitrogen, B represents -X'(CHSB2/SB)SBn/SBY', wherein n is an integer of 1 to 10, X' represents a functional group reactive with an activated ester and Y' represents an acid generating group having a pKa value of 4 or less, and RSB3/SBrepresents an alkylene group having 1 to 10 carbon atoms. PCOPYRIGHT: (C)2007,JPO&INPIT