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The selective enrichment of microbial metabolic characteristics of the desired

机译:选择性富集所需的微生物代谢特征

摘要

A concentration method for enzymes involved in the metabolism of the test substrate and / or selective enrichment of microorganisms that can metabolize the test substrate, the following steps: providing in a container a) microbial population, is initiated by b) an initial flow rate feed the solution into the vessel at a controlled flow rate, nutrient medium, and the solution, d includes a test substrate with at least a portion of the feed period, and provides a signal indicating the metabolic levels over the time frame of the c) concentration relates to a method for including as to provide an output based on a signal that can be evaluated for concentration of the enzymes produced by the microorganisms involved in the metabolism of the test substrate and / or selective enrichment of microorganisms to metabolize) test substrate. In order to facilitate the concentration process, when the steady state is detected, the condition can be set so as to increase the flow velocity in that period. When the condition, and preset conditions to suit the signal output to result in a change in fluid flow rate is appropriate, this may be achieved by varying the flow rate fed into the vessel solution. Conditions where previously set ranges of values ​​and a preset predetermined period, the period signals a predetermined within that range must remain.
机译:通过以下步骤进行浓缩酶的浓缩方法:参与测试底物代谢和/或选择性富集可代谢测试底物的微生物的酶:在容器中提供a)微生物种群,由b)初始流速进料开始d以受控的流速,营养培养基和溶液进入容器,溶液d包含至少一部分进料期的测试底物,并提供指示c)浓度时间范围内代谢水平的信号本发明涉及一种方法,其包括基于信号提供输出,该信号可以针对参与测试底物代谢和/或选择性富集微生物以代谢测试底物的微生物产生的酶的浓度进行评估。为了促进浓缩过程,当检测到稳态时,可以设置条件以增加该时段中的流速。当条件和适合信号输出以导致流体流速变化的预设条件合适时,可以通过改变送入容器溶液的流速来实现。预先设置值范围和预设预定时间段的条件,必须保留该范围内的预定时间段的信号。

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